Automated estimation of sodium, potassium, and calcium using the Eppendorf flame photometer

摘要

An Eppendorf flame photometer may be used to estimate sodium, potassium, and calcium in plasma which has been automatically sampled and diluted. Readings may be automatically recorded by feeding the output from the photomultiplier into a sensitive recorder. This method estimates 60 samples an hour, using approximately 0.12 ml. plasma per estimation. The accuracy of the method is discussed.